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本文提出的温州蜜柑萎缩病毒(SDV)提纯法,仅用100～200克病洋酸浆叶,且无需通过蔗糖密度梯度离心,在电镜下亦能看到中量直径约25nm 的球状病毒颗粒,粗提纯制剂OD_(860)/OD_(280)平均值为1.28,OD_(260)/OD_(240)平均值为1.15,初步显示出SDV 特征性紫外吸收曲线,提纯病毒产量约为4.5～11mg/kg病叶。比较证明,0.01M、PH7.0和0.1M、PH6.6的柠檬酸缓冲液以及0.5M、PH7.0的磷酸缓冲液是适宜的抽提缓冲液,洋酸浆是最好的繁殖寄主。
In this paper, the purification of satylanase from Wenzhou (SDV) is only about 100 ~ 200g, and without centrifugation by sucrose density gradient, it can also be seen in the medium volume of about 25nm in diameter under electron microscope, The average value of OD_ (860) / OD_ (280) was 1.28 and the average value of OD_ (260) / OD_ (240) was 1.15. The characteristic UV absorbance curve of SDV was preliminarily shown. The purified virus yield was about 4.5 ~ 11 mg / kg diseased leaves. It has been proved that 0.01 M, pH 7.0 and 0.1 M citrate buffer at pH 6.6 and 0.5 M phosphate buffer at pH 7.0 are the suitable extraction buffers. Physalis is the best breeding host.