在不孕门诊中提供冷冻保存精液十分必要,尤其获得性免疫缺陷综合征出现后,日益主张使用供者冷冻精液以替代新鲜精液。研究对供者精液建立有效的精液冷冻保存方法,以保证融冻后有高质量的精子。在1987年～1989年间对52名符合条件的供者精液进行分析。精液是在禁欲3天后手淫法取得,作全面精液分析,献精液标准为。容积2～6ml;精子浓度大于80×10~6/ml;活动力大于50％,绝大多数前向运动;正常形态精子大于45％。冷冻保存液每100ml中含15％甘油,20％卵黄,1.15g枸橼酸钠,1.8g葡萄糖,1g甘氨酸及青霉素10万IU,链霉素50mg,pH为7.3。每一容积的精液用32℃等量的冷冻保存液稀释后按规定方法在液氮蒸汽中冷却。精液的融冻在32℃进行,使用前复查精子活 It is necessary to provide cryopreserved semen in infertility clinics. Especially after the emergence of acquired immunodeficiency syndrome, it is increasingly advocated to use donor frozen semen instead of fresh semen. Study on donor semen to establish an effective sperm cryopreservation methods to ensure that there are high-quality sperm after thawing. Between 1987 and 1989 52 eligible donors were analyzed. Semen is obtained after masturbation 3 days of abstinence, for a comprehensive semen analysis, semen standard. Volume 2 ~ 6ml; sperm concentration greater than 80 × 10 ~ 6 / ml; activity greater than 50%, the vast majority of forward movement; normal form of sperm more than 45%. The cryopreservation solution contains 15% glycerol, 20% egg yolk, 1.15 g sodium citrate, 1.8 g glucose, 1 g glycine and penicillin 100,000 IU, streptomycin 50 mg, pH 7.3 per 100 ml. Each volume of semen diluted with an equal amount of cryopreservation fluid at 32 ° C is then cooled in liquid nitrogen steam as prescribed. Sperm freeze-thaw at 32 ℃, review the sperm before use live