松球壳孢菌菌落及孢子形态学对比研究

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通过 79个辽宁松球壳孢菌菌株与 4个A型和B型标准菌株、5个美国菌株在菌落和分生孢子形态方面的对比研究 ,发现辽宁的菌株在形态上基本一致。辽宁菌株与美国的类型A相近 ,主要表现在孢子表面平滑 ,通常无隔或有 1个隔 ,在成品PDA培养基上产生绒毛状、白色至灰色菌落。不同之处在于 ,辽宁菌株在自制PDA培养基上形成贴在培养基表面的菌落———与类型B相似 ,分生孢子长度介于A型和B型之间或与后者相近。 5个美国菌株与类型A一致。同时发现 ,培养基的来源对菌落形态研究非常重要 ;光照使菌落气生菌丝有所减少且出现绿色 ;培养基和寄主种类对孢子大小有影响。 Through the comparison of the colonies and conidia morphology of 79 strains of Pinus canadensis and 4 strains of type A and B and 5 strains of the United States in Liaoning Province, it was found that the strains of Liaoning were basically the same in morphology. The Liaoning strain is similar to type A in the United States and is mainly characterized by a smooth spore surface, usually without septa or septa, producing villous, white to gray colonies on the finished PDA medium. The difference is that the Liaoning strain formed colonies on the surface of the medium in a home-made PDA medium-similar to type B, with conidia length between or similar to type A and type B. Five U.S. strains were consistent with type A. At the same time, it was found that the source of culture medium was very important for colony morphology research. The illumination reduced the number of mycelia of green colony and appeared green. The medium and host species had influence on the spore size.
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