论文部分内容阅读
目的探讨大鼠膀胱组织中三型酸敏感离子通道(ASIC3)的表达与大鼠膀胱过度活动症(OAB)的关系。方法成年雌性大鼠膀胱造瘘后,随机分为对照组(0.9%氯化钠盐水腹腔注射)、OAB组(环磷酰胺腹腔注射)、干预组(OAB组基础上加入ASIC3抑制剂阿米洛利),每组20只。24h后对各组大鼠进行尿流动力学检测;取3组大鼠的膀胱组织,进行病理学检查;并通过免疫组织化学染色、RT-PCR和Western blot方法检测其ASIC3的表达。结果尿流动力学检测结果显示:对照组大鼠储尿期及排尿期未见明显不稳定收缩;与对照组相比,OAB组及干预组在储尿期可见不稳定收缩,即排尿间隔缩短(P<0.01),排尿次数增加(P<0.01)。干预组与OAB组相比,排尿间隔延长(P<0.05),排尿次数减少(P<0.05)。病理学检测提示:OAB组、干预组中大鼠膀胱黏膜破坏;免疫组化染色提示:大鼠膀胱组织有ASIC3表达,主要分布于膀胱黏膜;RT-PCR及Western blot提示:OAB组中膀胱黏膜上ASIC3基因及蛋白表达较对照组升高(P<0.01),加入ASIC3抑制剂后,干预组ASIC3基因及蛋白表达低于OAB组(P<0.05),但仍高于正常组(P<0.01)。结论大鼠膀胱组织有ASIC3基因及蛋白表达,OAB大鼠膀胱组织ASIC3基因及蛋白表达增高,ASIC抑制剂可抑制其表达,干预后,大鼠OAB症状减轻,说明膀胱组织ASIC3表达升高与膀胱逼尿肌反射亢进相关。
Objective To investigate the relationship between the expression of ASIC3 and the overactive bladder (OAB) in rat bladder tissue. Methods Adult female rats were randomly divided into control group (0.9% sodium chloride saline intraperitoneal injection), OAB group (cyclophosphamide intraperitoneal injection) and intervention group (OAB group) Lee), each group of 20. Urine flow cytometry was performed on rats in each group 24 hours later. The bladder tissues of 3 rats were examined by pathology. The expression of ASIC3 was detected by immunohistochemical staining, RT-PCR and Western blot. Results Urodynamics test results showed that there was no obvious unstable contraction in the control group during the period of urinary storage and urination. Compared with the control group, the unstable contraction of the OAB group and the intervention group during storage of the urine was observed, ie, the voiding interval was shortened P <0.01), urination frequency increased (P <0.01). Compared with the OAB group, the urination interval was prolonged in the intervention group (P <0.05) and the frequency of urination was decreased (P <0.05). Pathological examination indicated that the OAB group and the intervention group were damaged in the bladder mucosa. The expression of ASIC3 in the bladder tissue of the rats in the OAB group and in the intervention group was found to be mainly distributed in the bladder mucosa. RT-PCR and Western blot indicated that the bladder mucosa (P <0.01). After addition of ASIC3 inhibitor, the expression of ASIC3 gene and protein in the intervention group was lower than that in the OAB group (P <0.05), but still higher than that in the normal group (P <0.01) ). Conclusion The expression of ASIC3 gene and protein in the bladder tissue of rats was increased. The expression of ASIC3 gene and protein in the bladder tissue of OAB rats was increased. The inhibitor of ASICs could inhibit the expression of ASIC3 gene in OAB rats. After intervention, the expression of ASIC3 in bladder tissue was increased, Detrusor hyperreflexia related.