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目的 研究塔玛亚历山大藻（Alexandrium tamarens）的生长曲线及其在不同生长期分泌的麻痹性贝毒素（paralytic shelfish poisoning,PSP）的毒性大小,定量分析和比较冻融和超声波两种方法的破壁率及其优劣,为麻痹性贝毒提取方法的选择提供理论依据。方法 应用抽滤方法收集细胞,冻融和超声波两种方法进行破壁,对处于不同生长期的塔玛亚历山大藻进行细胞毒性检测和对比研究。结果 塔玛亚力山大藻的生长经历了延滞期（0～9d）、指数期（10～18d）和稳定期（19～22d）3个生长阶段。指数期细胞生长速度最快,然而,毒力最强的细胞是在培养时间为21d的稳定期,即9.43×10-6鼠单位/细胞（MU/cell）。经两种不同方法破碎后,延滞期细胞的毒性有很大差异,冻融后的细胞毒力比超声波破碎后的毒力大。此外,采用过滤方法收集细胞的回收率并不高（平均为69.0％）。结论 （1）在同一时期内,超声波破碎具有明显的优越性,但同时也具有降低细胞毒性的缺点;在相同生长条件和破壁条件下,延滞期细胞最容易破碎。（2）随着细胞培养时间的延长,细胞毒性逐渐增大,延滞期细胞的毒性己很高,随后的指数期和稳定期细胞的毒性只有少量增加。
Objective To study the growth curve of Alexandrium tamarens and the toxicity of paralytic shelf-life poisoning (PSP) secreted by different growth stages. Quantitative analysis and comparison of the two methods of freezing and thawing Rate and its advantages and disadvantages for the paralytic shellfish extraction method to provide a theoretical basis for the selection. Methods The cells were collected by suction filtration method, and the cells were broken by freeze-thaw and ultrasonic wave. Cytotoxicity and comparative studies were carried out on Alexandrium tamarense at different growth stages. Results The growth of A. tamarense had three growth stages of lag phase (0 ~ 9d), exponential phase (10 ~ 18d) and stable phase (19 ~ 22d). Exponential cells grew most rapidly, however, the most virulent cells were at a stationary phase of 21d, ie 9.43 × 10-6 MU / cell. After two different methods of crushing, the cytotoxicity of lag phase is very different, the cytotoxicity after freezing and thawing than the toxicity of ultrasonic crushing large. In addition, recovery of cells by filtration was not high (average of 69.0%). Conclusion (1) In the same period, ultrasound has obvious advantages in breaking, but also has the disadvantage of reducing cytotoxicity; under the same growth conditions and broken conditions, the cells are most likely to break the lag phase. (2) With the prolongation of cell culture time, the cytotoxicity gradually increased, the cytotoxicity of cells in the delaying phase had been very high, and the cell toxicity in exponential phase and stationary phase increased only a small amount.