目的研究类叶升麻苷对链尿佐菌素(STZ)侧脑室注射诱导的AD模型小鼠认知行为及突触可塑性的影响。方法将昆明小鼠和BALB/c鼠按体质量随机分为正常组、模型组、阳性对照组(Donepezil组)及类叶升麻苷低、中、高剂量组。除正常组注射PBS外,其余各组均侧脑室注射STZ(30μg/μL,5μL)制备AD模型。然后给予5、15、45mg/kg体质量类叶升麻苷溶液灌胃15d。给药完成后,利用自主活动、新物体识别、水迷宫等行为学实验测试小鼠的认知行为,通过突触长时程增强(LTP)观察类叶升麻苷对突触可塑性的影响。结果与模型组小鼠相比,类叶升麻苷给药组小鼠的认知行为及LTP提高,小鼠自主活动性增加,新物体识别优先指数升高,差异有统计学意义(P<0.05),在O迷宫中开臂区域的时间增加,差异有统计学意义(P<0.05),在水迷宫测试期逃避潜伏期缩短,差异有统计学意义(P<0.01),PS波增幅高于模型组,差异有统计学意义(P<0.05)。结论类叶升麻苷对链尿佐菌素(STZ)诱导的AD模型小鼠认知功能具有明显保护作用,其保护作用机制可能与类叶升麻苷可以提高小鼠海马PP-DG区的LTP和改善模型小鼠突触可塑性有关。 Objective To investigate the effect of acteoside on cognition and synaptic plasticity in AD model mice induced by streptozotocin (STZ) injection. Methods Kunming mice and BALB / c mice were randomly divided into normal group, model group, positive control group (Donepezil group) and low, medium and high doses of acteoside. Except the normal group, PBS was injected into the other groups, and all the other groups were injected intracerebroventricular injection of STZ (30μg / μL, 5μL) to prepare AD model. Then given 5,15,45 mg / kg body weight of the type of solution of sucralose gavage 15d. After administration, the cognitive behavior of mice was tested by behavioral experiments such as autonomous activity, new object recognition and water maze, and the effect of acteoside on synaptic plasticity was observed by long-term potentiation (LTP). Results Compared with the model mice, the cognitive behavior and LTP of the mice treated with acteosaclide increased, the activity of mice increased autonomously, and the priority index of new objects increased, with significant difference (P < 0.05). The time of opening the arm in the O-maze increased significantly (P <0.05), and the escape latency was shorter in the water maze test (P <0.01) Model group, the difference was statistically significant (P <0.05). CONCLUSION Acteoside has a significant protective effect on the cognitive function of AD model mice induced by streptozotocin (STZ), and its protective mechanism may be related to that acteoside can increase the expression of PP-DG in hippocampus of mice LTP is associated with improved synaptic plasticity in model mice.