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目的 通过计数细胞因子IFN γ和IL 10分泌细胞 ,检测多发性硬化 (MS)患者外周血单个核细胞IFN γ和IL 10分泌细胞水平 ,观察大剂量甲基强的松龙治疗对MS患者Th1及Th2细胞因子的影响。方法 将外周血单个核细胞暴露于中枢神经系统髓鞘素抗原髓鞘碱性蛋白进行体外短时间培养 ,用酶联免疫斑点试验(ELISPOT)检测IL 10和IFN γ分泌细胞 ,同时检测其他神经病组 (OND)及健康对照组。结果 显示MS患者IFN γ分泌细胞水平高于对照组 ,甲基强的松龙使MS患者IFN γ分泌细胞减少 ,对IL 10分泌细胞无明显影响。结论 MS患者存在Th1 Th2细胞因子的失衡 ,甲基强的松龙能抑制MSTh1类细胞因子IFN γ的产生 ,恢复其两类细胞因子之间的平衡而对MS患者产生治疗作用
Objective To detect the levels of IFNγ and IL-10 secreted by peripheral blood mononuclear cells in patients with multiple sclerosis (MS) by counting the cytokines IFNγ and IL-10 secreting cells. To observe the effect of high-dose methylprednisolone treatment on the levels of Th1 Th2 cytokines. Methods Peripheral blood mononuclear cells were exposed to myelin basic protein in central nervous system for short time culture in vitro. ELISPOT was used to detect the secretion of IL-10 and IFN-γ, and other neuropathies (OND) and healthy control group. The results showed that the level of IFN γ secreting cells in MS patients was higher than that in the control group. Methylprednisolone reduced the IFN γ secreting cells in MS patients and had no significant effect on IL 10 secreting cells. Conclusion There is an imbalance of Th1 cytokines in MS patients. Methylprednisolone can inhibit the production of IFN-γ, a MSTh1-like cytokine, and restore the balance between the two cytokines and have a therapeutic effect on MS patients