论文部分内容阅读
目的分析三七提取物(SE)对小鼠淋巴细胞体外活化、增殖以及对巨噬细胞产生NO的影响,探讨其免疫抑制作用的机制。方法以多克隆刺激剂刀豆蛋白A(Con A)刺激淋巴细胞活化和增殖,利用荧光标记的单克隆抗体双染技术和流式细胞术检测SE对小鼠CD3~+ T细胞CD69表达的影响;通过CPSE染色流式细胞术检测SE对淋巴细胞增殖的影响;用脂多糖(LPS)或淋巴细胞培养上清液(lymphocytes culture supernate,LCS)体外诱导小鼠腹腔巨噬细胞分泌NO,采用Griess试剂盒检测NO的水平。结果在Con A刺激6 h后小鼠T细胞活化率为59.79%,50、100μg/ml的SE可显著降低其活化率,分别为46.50%和37.73%(P<0.01)。在培养72 h后,不同浓度SE能明显抑制Con A诱导T细胞的增殖。SE在12.5～100μg/ml的浓度范围内对淋巴细胞培养上清和LPS诱导小鼠腹腔巨噬细胞NO的释放具有抑制作用(P<0.01)。结论三七提取物对小鼠淋巴细胞的活化、增殖有明显抑制作用,并能抑制巨噬细胞产生NO(P<0.01)。
Objective To analyze the effects of sanqi extract (SE) on the activation and proliferation of mouse lymphocytes in vitro and NO production in macrophages, and to explore the mechanism of immunosuppressive effects. Methods The activation and proliferation of lymphocytes were stimulated with the polyclonal stimulator concanavalin A (Con A). The effect of SE on CD69 expression in mouse CD3~+ T cells was detected by fluorescence double-labeled monoclonal antibody staining and flow cytometry. The effect of SE on lymphocyte proliferation was detected by CPSE staining and flow cytometry; intraperitoneal injection of lipopolysaccharide (LPS) or lymphocytes culture supernate (LCS) to induce NO secretion in mouse peritoneal macrophages was performed using Griess The kit detects NO levels. Results The activation rate of T cells was 59.79% after Con A stimulation for 6 h. SE of 50 and 100 μg/ml could significantly reduce the activation rate, 46.50% and 37.73%, respectively (P<0.01). After 72 h of culture, different concentrations of SE significantly inhibited Con A-induced T cell proliferation. SE had an inhibitory effect on lymphocyte culture supernatant and LPS-induced NO release in mouse peritoneal macrophages at a concentration range of 12.5-100 μg/ml (P<0.01). Conclusion Panax notoginseng extract can significantly inhibit the activation and proliferation of lymphocytes in mice and inhibit the production of NO in macrophages (P<0.01).