论文部分内容阅读
目的谷胱甘肽－Ｓ－转移酶Ｍ１和Ｔ１（ＧＳＴＭ１和ＧＳＴＴ１）空白基因型与肝癌遗传易感性的关系。方法应用多重ＰＣＲ技术检测６３例肝癌患者和８８例健康对照的ＧＳＴＭ１和ＧＳＴＴ１空白基因型。结果病例组ＧＳＴＭ１空白基因型的频率为５７．１％，对照组则为４２．０％，二者差异无显著性（χ２＝３．３５，Ｐ＝０．０６７），处于临界水平。ＯＲ值为１．８４（９５％ＣＩ＝０．９１～３．７３）。病例组ＧＳＴＴ１非空白基因型的频率为８７．３％，对照组则为６２．５％，二者差异有非常显著性（χ２＝１１．４２，Ｐ＝０．０００７２７４），ＯＲ值为４．１３（９５％ＣＩ＝１．６４～１０．７０）。叉生分析表明，ＧＳＴＴ１非空白基因型与肝癌的关联大于ＧＳＴＭ１空白基因型，两因素在肝癌发生中存在协同作用。结论具有ＧＳＴＭ１空白基因型和ＧＳＴＴ１非空白基因型的个体，患肝癌的危险性增加。
Objective To investigate the relationship between glutathione-S-transferase M1 and T1 (GSTM1 and GSTT1) genotypes and genetic susceptibility to HCC. Methods Multiplex PCR was used to detect GSTM1 and GSTT1 blank genotypes in 63 patients with liver cancer and 88 healthy controls. Results The frequency of blank genotype of GSTM1 was 57.1% in the case group and 42.0% in the control group. There was no significant difference between the two groups (χ2=3.35, P=0.067), which was at the critical level. The OR value was 1.84 (95% CI=0.91 to 3.73). The frequency of GSTT1 non-null genotype was 87.3% in the case group and 62.5% in the control group. The difference between the two groups was very significant (χ2=11.42, P=0.0007274), and the OR value was 4. 13 (95% CI = 1.64 to 10.70). Fork analysis showed that the association between GSTT1 non-white genotypes and HCC was greater than that of GSTM1 genotypes, and there were synergistic effects of the two factors in HCC development. Conclusion Individuals with a GSTM1 null genotype and a GSTT1 non-blank genotype have an increased risk of developing liver cancer.