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利用33对SSR引物对来自中国16个省的46份野生芒(Miscanthus sinensis)种质进行遗传多样性分析。结果显示:(1)33对SSR引物共扩增出87条DNA条带,75条为多态性条带,占86.21%,条带大小范围80～310 bp;(2)遗传多样性参数分析结果:Shannon’s信息指数(I)变幅为0.020～1.522,平均为0.745,引物多态性信息含量(PIC)变幅为0.040～0.738,平均为0.445,遗传相似系数(GS)的变幅为0.315～0.933,平均为0.569,说明我国芒种质资源遗传基础宽,遗传多样性丰富;(3)相似系数UMPGA聚类结果与主成分分析(PCA)结果一致,可将46份种质分为3大类群,类群Ⅰ主要由中部芒组成,类群Ⅱ主要由北方芒组成,类群Ⅲ主要由南方芒组成,西南芒在每个类群中均有渗透,这一结果说明芒种质资源的遗传分化与其种源的地理分布有一定的相关性,但与地理起源不能完全吻合。
The genetic diversity of 46 wild Miscanthus sinensis germplasms from 16 provinces in China was analyzed using 33 pairs of SSR primers. The results showed that: (1) 33 pairs of SSR primers amplified 87 DNA bands, 75 were polymorphic bands, accounting for 86.21%, and the bands ranged from 80 to 310 bp. (2) Genetic diversity analysis Results: The Shannon’s information index (I) ranged from 0.020 to 1.522 with an average of 0.745. The polymorphism information content (PIC) ranged from 0.040 to 0.738 with an average of 0.445. The genetic similarity coefficient (GS) ranged from 0.315 ~ 0.933, with an average of 0.569, indicating that the genetic basis of Mangliese germplasm resources in China is wide and the genetic diversity is abundant. (3) The results of UMPGA clustering with similar coefficients are consistent with the results of principal component analysis (PCA) Group Ⅰ, Group Ⅰ is mainly composed of the Central Mountain, Group Ⅱ is mainly composed of the North Mountain, Group Ⅲ is mainly composed of the Southern Mountain, Southwest Mountain in each group are infiltrated, the results show that the Mansland resources genetic differentiation and its provenance The geographical distribution of a certain degree of relevance, but not completely consistent with the geographical origin.