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目的:建立一去卵巢大鼠模型,通过运动和体外补充雌激素,探讨运动和雌激素对去卵巢大鼠脂肪代谢中GSK-3β、P-GSK-3β的影响。方法:以雌性去卵巢SD大鼠为研究对象,进行为期15周的实验,随机分为假手术安静组(Sham)、假手术运动组(Sham+E)、去卵巢安静组(OVX)、去卵巢运动组(OVX+E)和去卵巢雌激素组(OVX+E2)。运动组大鼠18 m/min,坡度5°,45 min/d,每周运动5 d;去卵巢激素组大鼠每周按体重25μg/kg注射E2,每周3次。实验结束后测定大鼠腹腔内脂肪重量,采用放射免疫方法测血清中雌激素E2水平,Western Blot测定大鼠脂肪总蛋白含量和脂肪组织GSK-3β和P-GSK-3β蛋白表达。结果:1)经过15周实验后,去卵巢安静组大鼠体重显著高于假手术安静组(P<0.05),运动和补充雌激素之后体重明显下降(P<0.05);2)去卵巢安静组大鼠腹腔内脂肪重量最大,运动和雌激素补充干预后脂肪重量明显降低(P<0.05);3)大鼠去卵巢之后脂肪组织中甘油三酯含量显著升高(P<0.05),运动和补充雌激素后又显著下降(P<0.05);4)运动训练后,假手术运动组大鼠和去卵巢运动组大鼠脂肪组织P-GSK-3β/GSK-3β蛋白表达量均显著升高(P<0.05)。结论:1)运动训练和体外补充雌激素,在一定程度上可以缓解去卵巢大鼠腹腔内脂肪的积累;2)中等强度运动训练能够提高去卵巢大鼠体内雌激素水平;3)运动能够上调脂肪组织中P-GSK-3β/GSK-3β蛋白的表达;体外补充雌激素对P-GSK-3β/GSK-3β蛋白的表达却无明显影响。
OBJECTIVE: To establish an ovariectomized rat model and study the effects of exercise and estrogen on GSK-3β, P-GSK-3β in ovariectomized rats by exercise and estrogen supplementation in vitro. Methods: Female ovariectomized SD rats were divided into sham operation group (Sham + E), ovariectomized group (OVX) and control group Ovary exercise group (OVX + E) and ovariectomized estrogen group (OVX + E2). Exercise group rats 18 m / min, slope 5 °, 45 min / d, exercise 5 d per week; ovariectomized rats were injected weekly E2 at a body weight of 25 μg / kg three times a week. At the end of the experiment, the weight of peritoneal fat in the rats was measured. The level of estrogen E2 in the serum was measured by radioimmunoassay. The content of total fat and GSK-3β and P-GSK-3β in adipose tissue were determined by Western Blot. The body weight of the ovariectomized rats was significantly higher than that of the sham-operated rats (P <0.05) after 15 weeks of treatment, and decreased significantly after exercise and estrogen supplementation (P <0.05). 2) The weight of fat in abdominal cavity of rats in group A was significantly higher than that in control group (P <0.05). 3) The content of triglyceride in adipose tissue of ovariectomized rats was significantly increased (P <0.05) (P <0.05); 4) After exercise training, the expression of P-GSK-3β / GSK-3β protein in the adipose tissue of rats in sham operation group and ovariectomized group increased significantly High (P <0.05). Conclusion: 1) exercise training and estrogen supplementation in vitro can alleviate adipose accumulation in the peritoneal cavity of ovariectomized rats to a certain extent; 2) moderate-intensity exercise training can increase the level of estrogen in ovariectomized rats; 3) exercise can increase The expression of P-GSK-3β / GSK-3β in adipose tissue was not significantly affected by estrogen supplementation in vitro.