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目的:探讨不同振动训练模式对大鼠骨骼肌细胞机械生长因子(MGF)mRNA表达和肌肉质量的影响。方法:42只雄性SD大鼠,根据对其施加的不同振动频率(35Hz、25Hz和15Hz)和持续时间(15min和5min),分为7组:安静对照组、低频率短时间振动组、低频率长时间振动组、中频率短时间振动组、中频率长时间振动组、高频率短时间振动组和高频率长时间振动组。各组进行相应振频和时间的振动训练,实验持续8周。实验结束后取材,检测腓肠肌质量相关指标、CK活性和肌细胞MGF mRNA表达水平。结果:各组大鼠腓肠肌质量无显著性差异,中频长时间组和高频短时间组腓肠肌指数(肌肉/体重比值)均显著高于安静对照组(P<0.05)。中频长时间组肌纤维横截面积(CSA)和CK水平均显著高于安静对照组(P<0.05)。各训练组大鼠骨骼肌MGF mRNA表达均显著高于安静对照组,中频长时间组显著高于除高频短时间组之外的其它训练组(P<0.05)。结果提示,适宜的振动(中频率长时间)训练有助于增加腓肠肌相对质量、肌纤维横截面积和肌细胞内CK活性,增强肌细胞MGFmRNA表达。
Objective: To investigate the effects of different vibration training modes on the expression of muscle growth factor (MGF) mRNA and muscle mass in rat skeletal muscle cells. Methods: Forty-four male Sprague-Dawley rats were divided into 7 groups according to different vibration frequencies (35Hz, 25Hz and 15Hz) and duration (15min and 5min): quiet control group, low frequency short time vibration group, Frequency long time vibration group, medium frequency short time vibration group, medium frequency long time vibration group, high frequency short time vibration group and high frequency long time vibration group. Each group was subjected to the vibration training of the corresponding frequency and time, the experiment lasted for 8 weeks. At the end of the experiment, the indexes of gastrocnemius muscle mass, CK activity and MGF mRNA expression were detected. Results: The gastrocnemius muscle mass of rats in each group had no significant difference. The gastrocnemius muscle index (muscle / body weight ratio) in long-term high-frequency group and short-term high frequency group were significantly higher than those in the quiet control group (P <0.05). The mid-frequency long-term muscle fiber cross-sectional area (CSA) and CK levels were significantly higher than the quiet control group (P <0.05). MGF mRNA expression of skeletal muscle in each training group was significantly higher than that in the quiet control group, and the long-term IF group was significantly higher than other training groups except the high-frequency and short-term group (P <0.05). The results suggest that appropriate vibration (medium frequency for a long time) training helps to increase the relative mass of gastrocnemius, muscle fiber cross-sectional area and intracellular CK activity, enhance muscle cell MGFmRNA expression.