论文部分内容阅读
目的建立水中呋喃丹的柱前衍生-高效液相色谱测定法。方法水样经高效液相色谱柱前衍生,采用荧光检测器(激发光波长:339 nm;发射光波长:445 nm),色谱柱为DIKMA C18(150 mm×4.6 mm,5μm),流动相为甲醇-磷酸二氢钾(体积比为60∶40),流量为1.0 ml/min,柱温为室温。结果该方法标准曲线回归方程为y=49.995x-0.240,相关系数为0.999 8,检出限为0.125μg/L。平均加标回收率为90.2%~94.5%,相对标准偏差(RSD)为3.98%~4.63%。结论该方法操作简便、快速,试剂用量小,适合于水中呋喃丹的含量检测。
Objective To establish a pre-column derivatization-high performance liquid chromatography (HPLC) assay for carbofuran in water. Methods The sample was derivatized with high performance liquid chromatography (HPLC) using a fluorescence detector (excitation wavelength: 339 nm and emission wavelength: 445 nm). The column was DIKMA C18 (150 mm × 4.6 mm, 5 μm) Methanol-potassium dihydrogen phosphate (volume ratio of 60:40), the flow rate of 1.0 ml / min, the column temperature was room temperature. Results The calibration curve of this method was y = 49.995x-0.240, the correlation coefficient was 0.999 8, the detection limit was 0.125μg / L. The average recovery was 90.2% ~ 94.5% and the relative standard deviation (RSD) was 3.98% ~ 4.63%. Conclusion The method is simple and rapid, the reagent dosage is small, suitable for the determination of carbofuran in water.