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Objective:We investigated the effects of exogenous AP-2α gene on SW620 cell cycle,apoptosis and prolifera.tion.Methods:The Plasmid pcDNA3.1(+)-AP-2a was transfectecl into colorectal carcinoma SW620 cells line by liposome me-diation for transient expression.After AP-2α transfected SW620 cells,the exogenous AP-2a mRNA and protein express were determined by the method of Real-time PCR and Western blot.In order to elucidate the effect of expression of exogenous AP-2α gene on the colorectal cancer cell SW620,the proliferation rates were analyzed by growth curves for cells including SW620,pcDNA3.1(+)/SW620 and pcDNA3.1(+)-AP-2α/SW620.At same time,the apoptotic rate of cells and the cell cycle analysis were also tested by flow cytometry.Results:The mRNA and protein expressions of AP-2α gene could be enhanced by transfecting of pcDNA3.1(+)-AP-2α recombinant plasmid into SW620 call.The cell growth rates of SW620 cells transfected with pcDNA3.1(+)/AP-2α were slower than those transfected with pcDNA3.1(+) or untransfected.The apoptotic rates were increased compared with pcDNA3.1(+)/SW620 and SW620 (P＜0.05),which indicated that over-expression of AP-2α gene could efficiently inhibit the growth of SW620 cells and induce apoptosis.FCM analyses indicated that the cells being arrested in G1 phase.Conclusion:AP-2α gene can be efficiently transfected into SW620 cells and over-expression AP-2α protein in the transfected cells.AP-2α induces G1 arrest and apoptosis,suppressive effect on cell growth.