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目的探讨T2DM患者不同血糖控制水平葡萄糖转运蛋白1/4(GLUT1/4)的表达及其对迁移抑制因子(MIF)的影响。方法选取T2DM患者84例(研究组)及正常健康成志愿者70名(NC组)。根据HbA1c水平将研究组再分为低水平亚组(LH)29例(HbA1c<7.0%);中水平亚组(MH)28例(7.0%≤HbA1c<10.0%);高水平亚组(HH)27例(HbA1c≥10.0%)。ELISA检测各组血浆GLUT1/4、MIF及FPG等相关指标;高效液相色谱法测定HbA1c水平;RT-PCR测定GLUT1/4、MIF表达情况。多元回归分析GLUT4与MIF、HbA1c的相关性。结果研究组FPG、2hPG、FIns与NC组比较,差异有统计学意义(P<0.05),ELISA与RT-PCR结果发现,研究组GLUT1与NC组比较,差异无统计学意义(P>0.05)。HH、MH亚组HbA1c、FIns、HOMA-IR、2hPG、TNF-α、IL-6、细胞间黏附因子-1(ICAM-1)、MIF及GLUT4均高于LH亚组(P<0.05),HH亚组HbA1c、FIns、TNF-α、IL-6、ICAM-1、高敏C-反应蛋白(hsC-RP)、MIF及GLUT4高于MH亚组(P<0.05)。随着HbA1c水平的升高,各亚组GLUT4下降(P<0.05)。Logistic回归分析发现,MIF、HbA1c是GLUT4的独立危险因素。结论HbA1c、MIF在T2DM中呈高表达,其与GLUT1无相关性,而与GLUT4呈负相关。
Objective To investigate the expression of glucose transporter 1/4 (GLUT1 / 4) and its effect on migration inhibition factor (MIF) in patients with T2DM. Methods Totally 84 patients with T2DM (study group) and 70 normal healthy volunteers (NC group) were selected. According to the level of HbA1c, the study group was further subdivided into low level subgroup (LH) of 29 cases (HbA1c <7.0%); middle level subgroup (MH) of 28 cases (7.0% ≤HbA1c <10.0%); ) 27 cases (HbA1c≥10.0%). Serum GLUT1 / 4, MIF and FPG were measured by ELISA. The level of HbA1c was determined by high performance liquid chromatography. The expression of GLUT1 / 4 and MIF was detected by RT-PCR. Multiple regression analysis of GLUT4 and MIF, HbA1c correlation. Results The results of ELISA and RT-PCR showed that there was no significant difference in GLUT1 and NC between the two groups (P> 0.05). There was no significant difference between FP group, 2hPG group and FIns group and NC group (P <0.05) . The levels of HbA1c, FIns, HOMA-IR, 2hPG, TNF-α, IL-6, ICAM-1, MIF and GLUT4 in HH and MH subgroups were higher than those in LH subgroups (P < The levels of HbA1c, FIns, TNF-α, IL-6, ICAM-1, hsC-RP, MIF and GLUT4 in HH subgroup were higher than those in MH subgroup (P <0.05). With the increase of HbA1c, the GLUT4 in each subgroup decreased (P <0.05). Logistic regression analysis found that MIF and HbA1c were independent risk factors of GLUT4. Conclusion HbA1c and MIF are highly expressed in T2DM, but not related to GLUT1, but negatively correlated with GLUT4.