为选育具有稻瘟病持久抗性的水稻新品系提供可利用资源,1993 年以小粒野生稻为外源DNA供体,采用花粉管通道法,将其稻瘟病抗性导入栽培稻24D中。经过4 年连续6 代的选择,成功地将小粒野生稻稻瘟病抗性转移到栽培稻中,获得了9 份苗叶瘟和穗颈瘟均得到改良的抗性材料。因此,利用分子育种技术改良水稻抗病性是可行的。 In order to breed new rice lines with long-lasting resistance to blast, the available resources were provided. In 1993, small-sized wild rice (Oryza sativa L.) was used as exogenous DNA donor and introduced into rice 24D by pollen tube channel method. After four years of continuous selection for six generations, the blast resistance of small-particle wild rice was successfully transferred to the cultivated rice, and 9 resistant materials with improved leaf-seedling blast and panicle neck blast were obtained. Therefore, the use of molecular breeding techniques to improve rice disease resistance is feasible.