重组人生长激素的质量鉴定

来源 :JOURNAL OF CHINESE PHARMACEUTICAL SCIENCE | 被引量 : 0次 | 上传用户:talen
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用SDS─聚丙烯酰胺凝胶电泳、等电聚焦、反相液相色谱、分子排阻液相色谱、疏水相互作用液相色谱。胰肽图谱。生物测定、斑点免疫印迹。酶联免疫吸附和地高辛标记DNA等多种手段,对国产重组人生长激素进行全面质量鉴定并与国外同类产品进行比较,其结果表明:国产重组人生长激素的质量基本达到国外同类产品的水平,为DNA重组药物的质控积累了重要数据。 SDS - polyacrylamide gel electrophoresis, isoelectric focusing, reverse phase liquid chromatography, molecular exclusion liquid chromatography, hydrophobic interaction liquid chromatography. Pancreatic peptide map. Bioassay, dot blotting. Enzyme-linked immunosorbent assay and digoxin-labeled DNA and other means to conduct a comprehensive quality identification of domestic recombinant human growth hormone and compared with similar foreign products, the results show that: the quality of domestic recombinant human growth hormone basically reached the same foreign products Level, accumulating important data for the quality control of DNA recombinant drugs.
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