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目的 :探讨粘蛋白 1(MUC1)表达与河南食管癌高发区食管鳞状细胞癌 (SCC)、贲门腺癌 (GCA)细胞分化程度与淋巴结转移间的关系。方法 :免疫组化ABC法检测MUC1在 5 0例SCC和 2 1例GCA及配对淋巴结组织中的表达 ,分别以活检正常的 10例食管粘膜组织及 8例贲门粘膜组织为对照。结果 :MUC1免疫阳性染色呈棕黄色或深棕黄色。 4 8例SCC(48/5 0 )和 2 1例GCA(2 1/2 1)原发病灶中均有不同程度的MUC1表达 ,6例正常食管粘膜 (6 /10 )和 4例正常贲门粘膜 (4/8)中观察到MUC1弱免疫阳性反应。正常细胞中 ,MUC1主要表达于细胞膜近管腔或腺腔侧 ,但在癌细胞中则胞膜和胞浆中均有表达。SCC与正常食管粘膜、GCA与正常贲门粘膜MUC1免疫阳性反应强度间差异有统计学意义 (分别为P =0 .0 0 5 0 6 ,P =0 .0 0 2 95 ) ,SCC和GCA原发病灶与转移淋巴结间MUC1的一致性表达差异均有统计学意义 (分别为U =4 .4 15 ,P <0 .0 0 0 1,U =2 .2 74 ,P <0 .0 5 ) ,一致性MUC1变化的发生率在SCC为 6 0 % (30 /5 0 ) ,GCA为 85 .71% (18/2 1) ;其中一致性阳性的发生率在SCC为 5 6 % ,GCA为10 0 %。SCC组织分化的恶性程度与MUC1免疫反应的强度呈负相关 (r =- 0 .35 6 6 ,P <0 .0 5 ) ,GCA出现类似趋势 ,但无统计学意义 (P >0 .0 5 )。MUC1免疫反?
Objective: To investigate the relationship between mucin 1 (MUC1) expression and differentiation of lymph node metastases in esophageal squamous cell carcinoma (SCC) and cardial adenocarcinoma (GCA) cells in high incidence areas of esophageal cancer in Henan Province. METHODS: Immunohistochemical ABC method was used to detect the expression of MUC1 in 50 cases of SCC and 21 cases of GCA and matched lymph node tissues. The normal biopsy of 10 cases of esophageal mucosa and 8 cases of cardiac mucosa were used as controls. Results : MUC1 immunopositive staining was brownish yellow or dark brownish yellow. 4 cases of SCC (48/5 0) and 21 cases of GCA (2 1/2 1) had different degrees of expression of MUC1, 6 cases of normal esophageal mucosa (6/10) and 4 cases of normal cardiac mucosa. MUC1 weak immunopositive reaction was observed in (4/8). In normal cells, MUC1 is mainly expressed in the proximal lumen of the cell membrane or the lumen of the gland, but is expressed in both the cell membrane and cytoplasm in cancer cells. There was a statistically significant difference in the intensity of MUC1 immunoreactivity between SCC and normal esophageal mucosa, GCA and normal cardiac mucosa (P = 0.050, P = 0.02026, respectively). SCC and GCA primary The differences in the consistent expression of MUC1 between lesions and metastatic lymph nodes were statistically significant (U = 4.4 15, P <0 0 0 1, U = 2.27, P <0.05, respectively). The incidence of consistent MUC1 changes was 60% (30/50%) in SCC and 85.71% (18/2 1) in GCA; the incidence of consistent positive was 56% in SCC and 10 in GCA. 0 %. The malignancy of SCC tissue differentiation was negatively correlated with the intensity of MUC1 immune response (r = -0.356, P < 0.05). GCA showed a similar trend, but it was not statistically significant (P > 0.05). ). MUC1 immune response?