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包括血管内皮细胞在内的多种细胞和组织存在腺苷三磷酸双磷酸酶（ａｐｙｒａｓｅ） ， 但心内膜内皮细胞是否含有ａｐｙｒａｓｅ 尚无报道。本文旨在研究牛心内膜内皮细胞ａｐｙｒａｓｅ 的特性。以无机磷释放法检测培养牛心内膜内皮细胞（ＢＥＥＣ） ａｐｙｒａｓｅ 的活性。公认的ａｐｙｒａｓｅ 抑制剂叠氮钠呈浓度依赖性地抑制ａｐｙｒａｓｅ 活性； 另一种ａｐｙｒａｓｅ 抑制剂氟化钠（２０ ｍｍｏｌ／Ｌ） 也明显抑制ａｐｙｒａｓｅ 活性。而Ｎａ＋ ／Ｋ＋ＡＴＰａｓｅ 的抑制剂哇巴因（３ ｍｍｏｌ／Ｌ） 却不能抑制该酶活性。ＢＥＥＣａｐｙｒａｓｅ 活性依赖于钙或镁等二价阳离子以及ｐＨ 的改变， ＥＤＴＡ 或ＥＧＴＡ （１ｍｍｏｌ／Ｌ） 均能完全抑制其活性， 在ｐＨ 值为８－５ 时活性最高。由此可见， 牛心内膜内皮细胞存在叠氮钠敏感的、依赖于二价阳离子和ｐＨ 值的腺苷三磷酸双磷酸酶活性。
There are apyrase in a variety of cells and tissues, including vascular endothelial cells, but whether endocardial endothelial cells contain apyrase has not been reported yet. This article aims to investigate the properties of apyrase in bovine endocardial endothelial cells. The activity of apyrase in bovine endocardial endothelial cells (BEEC) was detected by inorganic phosphorus release assay. Sodium azide, a recognized apyrase inhibitor, inhibited apyrase activity in a concentration-dependent manner. Another apyrase inhibitor, sodium fluoride (20 mmol / L), also significantly inhibited apyrase activity. But Na + / K + ATPase inhibitor ouabain (3 mmol / L) did not inhibit the activity of this enzyme. BEECapyrase activity depends on divalent cations such as calcium or magnesium and changes in pH, EDTA or EGTA (1mmol / L) can completely inhibit its activity, the highest activity at a pH of 8-5. Thus, bovine endocardial endothelial cells exist sodium azide sensitive, dependent on the divalent cation and pH of the ATPase activity of adenosine triphosphate.