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目的探讨负载人卵巢癌细胞株SKOV3冻融抗原(Ag)的树突状细胞(DC)与细胞因子诱导的杀伤细胞(CIK)共培养后对SKOV3杀伤作用的影响。方法取12例卵巢癌患者外周血,常规诱导出DC及CIK细胞,以SKOV3冻融Ag负载DC,经Ag负载与未经Ag负载的DC分别和CIK细胞共培养,流式细胞技术分析DC、Ag-DC、Ag-DC-CIK3组DC细胞表型,CIK、DC-CIK、Ag-DC-CIK组CIK细胞表型;乳酸脱氢酶释放法测CIK组、DC-CIK组、Ag-DC-CIK组对SKOV3杀伤活性。结果Ag-DC-CIK组中DC细胞成熟表型高于DC及Ag-DC组(P<0.01);Ag-DC-CIK组中CIK细胞成熟表型高于CIK及DC-CIK组(P<0.01);Ag-DC-CIK组对SKOV3杀伤率高于CIK及DC-CIK组(P<0.01)。结论负载SKOV3冻融Ag的DC与CIK共培养可促进DC、CIK的成熟,且可提高对SKOV3的杀伤作用。
Objective To investigate the effects of co-cultured with dendritic cells (DCs) loaded with human ovarian cancer cell line SKOV3 and cytokine-induced killer cells (CIK) on the cytotoxicity of SKOV3. Methods Peripheral blood was obtained from 12 patients with ovarian cancer. DC and CIK cells were routinely induced. DCs were loaded with SKOV3 by freeze-thawing Ag, DCs were co-cultured with CIK cells by Ag and DC without Ag, respectively. Flow cytometry was used to analyze DCs, The phenotypes of CIK cells in CIK, CIK, DC-CIK and Ag-DC-CIK groups were detected by flow cytometry, Ag-DC and Ag- -CIK group on SKOV3 killing activity. Results The maturation phenotypes of DCs in Ag-DC-CIK group were higher than those in DC and Ag-DC groups (P <0.01). The maturation phenotypes of CIK cells in Ag-DC-CIK group were higher than those in CIK and DC-CIK groups 0.01). The killing rate of SKOV3 in Ag-DC-CIK group was higher than that in CIK and DC-CIK groups (P <0.01). Conclusions DC co-cultured with CIK loaded with SKOV3 freeze-thaw Ag could promote the maturation of DC and CIK and enhance the killing effect on SKOV3.