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目的研究野菊花舒张血管及抗炎机制,同时探索其活性物质基础。方法通过离体血管环张力实验观察野菊花提取物及其主要单体对大鼠胸主动脉环经苯肾上腺素引起血管环收缩的影响。以Griess反应检测野菊花提取物及其单体对LPS(1μg/ml)/IFN-γ(10U/ml)刺激巨噬细胞RAW264.7释放NO的抑制作用;噻唑兰(MTT)比色法检测二者对细胞活力的影响;western blot检测二者对诱导型一氧化氮合酶(iNOS)及环氧合酶-2(COX-2)的抑制作用。结果野菊花提取物、木犀草素对血管的舒张效应为部分内皮依赖性、部分非内皮依赖性;二者对均能剂量依赖性地抑制炎症巨噬细胞生成NO、抑制iNOS表达,同时无细胞毒性,对COX-2表达无影响。结论野菊花提取物的血管舒张效应,抑制炎症细胞内NO及其诱导型合酶iNOS表达可能是其药理作用机制。
Objective To study the mechanism of relaxation and anti-inflammatory of wild chrysanthemum and explore the basis of its active substance. Methods The in vitro vascular ring tension test was used to observe the effect of wild chrysanthemum extract and its main monomers on the contraction of vasculature induced by phenylephrine in the thoracic aorta. Griess reaction was used to detect the inhibitory effect of wild chrysanthemum extract and its monomer on the release of NO from macrophages RAW264.7 stimulated by LPS (1μg / ml) / IFN-γ (10U / ml); MTT assay The effect of both on the cell viability was examined by western blot. The inhibitory effects of both on iNOS and COX-2 were examined by western blot. Results The vasodilatation effect of wild chrysanthemum extract and luteolin was partly endothelium-dependent and partly non-endothelium-dependent. Both of them could inhibit NO production and inhibit iNOS expression in inflammatory macrophages in a dose-dependent manner, Toxicity, no effect on COX-2 expression. Conclusions Vasodilator effect of Chrysanthemum indicum extract and inhibition of NO and iNOS expression in inflammatory cells may be the mechanism of its pharmacological action.