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目的:观察助脾散精方(ZPSJ)对胰岛抵抗(IR)模型大鼠胰岛素信号通路相关蛋白胰岛素受体(InsR)、胰岛素受体底物-1(IRS-1)及胰岛素降解酶(IDE)的影响。方法:SD大鼠尾iv链脲佐菌素(STZ)加高糖高脂饮食复制IR模型,分为正常组、模型组、罗格列酮组(罗格列酮7.2×10-4g.kg-1.d-1)和ZPSJ治疗组(含生药6.41 g.kg-1.d-1)。结果:IR模型组大鼠海马CA1区InsR,IRS-1阳性表达的神经元明显增多,ZPSJ治疗组,InsR,IRS-1表达明显减少(P<0.01)。IR模型组大鼠海马CA1区IDE阳性表达的神经元明显减少,ZPSJ治疗组IDE表达明显增加,与模型组比较有显著性差异(P<0.01)。结论:ZPSJ方能减少IR模型大鼠海马组织InsR,IRS-1的表达,同时增加海马组织IDE的表达。
OBJECTIVE: To observe the effect of ZBPX on Insulin, Insulin Receptor Substrate-1 (IRS-1) and Insulin-Degrading Enzyme (IDE) in rat insulin resistance (IR) )Impact. Methods: IR model was made by intraperitoneal injection of streptozotocin (STZ) plus high glucose and high fat diet in SD rats. The rats were divided into normal group, model group, rosiglitazone group (rosiglitazone 7.2 × 10-4g.kg -1.d-1) and ZPSJ treatment group (crude drug containing 6.41 g.kg-1.d-1). Results: Insulin and IRS-1 positive neurons in hippocampal CA1 area were significantly increased in IR group, while the expression of InsR and IRS-1 in ZPSJ treatment group was significantly decreased (P <0.01). Compared with model group, the expression of IDE in hippocampal CA1 area of IR model group was significantly decreased, while the expression of IDE in ZPSJ treatment group was significantly increased (P <0.01). Conclusion: ZPSJ can reduce the expression of InsR and IRS-1 in hippocampus of IR rats and increase the expression of IDE in hippocampus.