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目的研究莱菔子水提液中芥子碱硫氰酸盐在大鼠小肠的吸收特性。方法采用大鼠在体单向肠灌流模型,用HPLC法对芥子碱硫氰酸盐质量浓度进行分析,计算其吸收速率常数和表观吸收系数,分别研究药物质量浓度和P-糖蛋白(P-gp)抑制剂对芥子碱硫氰酸盐吸收的影响。结果芥子碱硫氰酸盐在大鼠小肠的吸收速率常数(Ka)和表观吸收系数(Papp)随浓度增加先增加后减小,且芥子碱硫氰酸盐的表观吸收速率小于1.8伊10-4cm·min-1,P-gp抑制剂对芥子碱硫氰酸盐的吸收具有显著影响(P<0.01)。结论芥子碱硫氰酸盐在大鼠小肠中的吸收机制可能为主动转运或易化扩散,且其在小肠中的吸收较差,芥子碱硫氰酸盐为P-gp底物。
Objective To study the absorption characteristics of sinapine thiocyanate in the small intestine of Radish extracts. Methods The model of rat intestinal perfused rat was established. The concentration of sinapine thiocyanate was analyzed by HPLC. The absorption rate constant and apparent absorption coefficient were calculated. The concentrations of P-glycoprotein (P -gp) Inhibitors on the Absorption of Sinapic thiocyanate. Results The adsorption rate constant (Ka) and apparent absorption coefficient (Papp) of sinapine thiocyanate increased firstly and then decreased with the increasing of concentration, and the apparent absorption rate of sinapine thiocyanate was less than 1.8% 10-4cm · min-1, P-gp inhibitor had a significant effect on the absorption of sinapine thiocyanate (P <0.01). Conclusions The mechanism of absorption of sinapine thiocyanate in rat intestine may be active transport or facilitated diffusion, and its absorption in the small intestine is poor, and sinapine thiocyanate is P-gp substrate.