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目的：探讨细胞周期蛋白Ｄ１（ｃｙｃｌｉｎＤ１）的表达与喉鳞癌（ＳＣＣＬ）的关系。方法：对３５例经病理证实ＳＣＣＬ患者的肿瘤组织及其邻近的正常粘膜，运用反转录多聚酶链反应（ＲＴ－ＰＣＲ）、光密度半定量法，分别检测其ｃｙｃｌｉｎＤ１的表达程度。结果：发现几乎所有的肿瘤标本的的表达量要高于正常粘膜（Ｐ＜０．０５），有淋巴结转移者的表达量要明显高于无淋巴结转移者（Ｐ＜０．０５）；表达量与ＴＭＮ分期也有明显的相关性（Ｐ＜０．０１）；但与病理分级无关（Ｐ＞０．０５）。在性别和年龄分布上表达的程度也无差异。结论：ｃｙｃｌｉｎＤ１表达程度的检测技术可能成为判断ＳＣＣＬ患者的治疗效果及预后估计的提示性依据。
Objective: To investigate the relationship between the expression of cyclin D1 and laryngeal squamous cell carcinoma (SCCL). Methods: The expression of cyclinD1 was detected by reverse transcription-polymerase chain reaction (RT-PCR) and semi-quantitative optical density in 35 cases of pathologically confirmed tumor tissue and its adjacent normal mucosa. Results: It was found that the expression of all the tumor samples was higher than that of the normal mucosa (P <0.05), and the expression of lymph node metastasis was significantly higher than those without lymph node metastasis (P <0.05) There was also a significant correlation with TMN staging (P <0.01), but not with pathological grade (P> 0.05). There is no difference in the degree of gender and age distribution. Conclusion: The detection of cyclinD1 expression level may be a suggestive basis for judging the therapeutic effect and prognosis of patients with SCCL.