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目的:探讨胎盘转化生长因子-β(TGF-β)三种亚型及其受体的表达与妊娠期肝内胆汁淤积症(ICP)的关系。方法:用免疫组织化学染色技术(SP法)检测ICP患者和正常妊娠胎盘组织中TGF-β1、2、3三种亚型和两种功能型受体(TGF-βRⅠ和TGF-βRⅡ)的表达水平。结果:ICP患者胎盘绒毛TGF-β2表达的平均面积、染色强度和IOD值均明显小于正常妊娠组(P<0.01),其IOD值与母血TBA呈负相关(r=-0.824,P<0.01);TGF-βRⅡ的表达面积和IOD值高于正常妊娠组(P<0.01,P<0.05);而TGF-β1、3和TGF-βRⅠ与正常相似。结论 :ICP患者存在免疫耐受-免疫排斥不平衡的原因可能与TGF-β2/TGF-βRⅡ表达异常有关。
Objective: To investigate the relationship between the expression of three subtypes of transforming growth factor-β (TGF-β) and their receptors in placenta and intrahepatic cholestasis of pregnancy (ICP). Methods: The expression of TGF-β1,2,3 and two functional receptors (TGF-βRⅠ and TGF-βRⅡ) in ICP and normal pregnancy placenta were detected by immunohistochemical staining (SP method) Level. Results: The average area, staining intensity and IOD value of TGF-β2 in placenta of ICP patients were significantly lower than those of normal pregnant women (P <0.01), and the IOD values were negatively correlated with TBA of pregnant women (r = -0.824, P <0.01) ); The expression area and IOD value of TGF-βRⅡwere higher than that of normal pregnancy group (P <0.01, P <0.05), while TGF-β1,3 and TGF-βRⅠwere similar to normal. Conclusion: The reason of imbalance of immunological tolerance and immune rejection in ICP patients may be related to the abnormal expression of TGF-β2 / TGF-βRⅡ.