应用硫酸铵沉淀及亲和层析法由单纯疱疹病毒Ⅰ型感染的BHK细胞分离、纯化病毒诱导的脱氧胸苷激酶。对纯化酶的泳动率,磷酸供体的亲和力,抑制剂及抗生素的敏感性进行了研究. Virus-induced deoxythymidine kinase was isolated and purified by ammonium sulfate precipitation and affinity chromatography from herpes simplex virus type 1 BHK cells. The mobility of purified enzyme, affinity of phosphate donor, inhibitor and antibiotic sensitivity were studied.