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目的:制备载转化生长因子β1(TGF-β1)的壳聚糖-聚己内酯微球并研究其体外缓释行为。方法:以三聚磷酸钠为交联剂,利用水/油乳液方法制备载TGF-β1因子的壳聚糖-聚己内酯微球。利用酶联免疫吸附方法(ELISA)检测壳聚糖-聚己内酯微球的体外缓释行为。结果:在目前制备条件下,壳聚糖-聚己内酯微球具有好的形态,平均粒径可有效控制在10-20μm范围内。壳聚糖-聚己内酯中的聚己内酯含量能有效控制微球的初始释放和随后的缓释行为。优化获得的壳聚糖-聚己内酯微球能够以不同的速率控制TGF-β1持续释放超过4周。结论:载TGF-β1因子壳聚糖-聚己内酯微球的制备工艺稳定和可控,其初始释放和持续缓释行为可以由壳聚糖-聚己内酯中聚己内酯的含量有效调控。
OBJECTIVE: To prepare chitosan-polycaprolactone microspheres containing transforming growth factor β1 (TGF-β1) and study its in vitro sustained release behavior. Methods: Chitosan-polycaprolactone microspheres containing TGF-β1 were prepared by water / oil emulsion using sodium tripolyphosphate as crosslinking agent. In vitro enzyme-linked immunosorbent assay (ELISA) was used to detect the sustained release of chitosan-polycaprolactone microspheres. Results: Under the current preparation conditions, the chitosan-polycaprolactone microspheres have a good morphology and the average particle size can be effectively controlled in the range of 10-20 μm. The content of polycaprolactone in chitosan-polycaprolactone can effectively control the initial release and subsequent release of microspheres. The optimized chitosan-polycaprolactone microspheres were able to control the sustained release of TGF-β1 for more than 4 weeks at different rates. CONCLUSION: The preparation of TGF-β1-loaded chitosan-polycaprolactone microspheres is stable and controllable. The initial release and sustained release of chitosan-polycaprolactone microspheres can be determined by the content of polycaprolactone Effective regulation.