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目的:研究肝细胞生长因子受体c-Met在口咽鳞癌中的预后价值。方法:运用一种新的原位蛋白自动定量分析法,研究由108例口咽鳞癌组成的组织微阵列中c-Met抗体染色的形式和蛋白定量表达。结果:c-Met高细胞核染色的肿瘤患者,相对低染色组,其局部复发率明显上升(77%:37%),5年无瘤生存率显著性下降(8%:33%),而且其总体生存率明显下降(8%:35%)。多变量风险回归分析中,c-Met细胞核内的表达状况无论对5年复发率、无瘤生存率和总体生存率都是最有意义的预测因子。但细胞质表达的c-Met与肿瘤的预后不存在关联性。另外,对于c-Met高细胞核表达的肿瘤患者来说,其临床治愈率较低(63%:91%)。细胞质表达的c-Met与MAPK表达密切相关(P<0.001),而细胞核表达的c-Met无此相关性。结论:细胞核内c-Met表达与口咽鳞癌的预后不良相关,这种c-Met的表达方式可能是激发了侵袭性肿瘤的替代传导通路所造成的。
Objective: To investigate the prognostic value of hepatocyte growth factor receptor c-Met in oropharyngeal squamous cell carcinoma. Methods: The expression of c-Met antibody in tissue microarray consisted of 108 cases of oropharyngeal squamous cell carcinoma was studied by using a new automated in situ protein quantification method. Results: The local recurrence rate (77%: 37%) and the 5-year disease free survival rate decreased significantly (8%: 33%) in patients with c-Met high cell nuclear staining and relatively low staining The overall survival rate decreased significantly (8%: 35%). Multivariate risk regression analysis showed that the expression of c-Met in the nucleus was the most significant predictor of 5-year recurrence, disease-free survival, and overall survival. However, there is no correlation between cytoplasmic c-Met expression and tumor prognosis. In addition, the clinical cure rate was lower for patients with tumors with c-Met high-nuclear expression (63%: 91%). Cytoplasmic c-Met was closely related to MAPK expression (P <0.001), but not to nuclear c-Met expression. Conclusion: The expression of c-Met in the nucleus is related to the poor prognosis of oropharyngeal squamous cell carcinoma. The expression of c-Met may be caused by the alternative conduction pathways that stimulate aggressive tumors.