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DREB类转录因子是一类植物中特有的,在非生物逆境胁迫中起重要作用的调控因子。本研究以割手密为材料,采用同源基因克隆的方法克隆获得2个DREB2类转录因子基因Ss DREB2-1和Ss DREB2-2(Gen Bank登录号分别为KU963264和KU963265)。序列分析表明,这2个基因的长度分别为814 bp和709 bp,分别编码262和227个氨基酸;预测其蛋白质分子量分别为28.66 k D和24.95 k D,等电点(PI)分别为5.42和6.47。氨基酸亲水/疏水性分析表明,这2个转录因子属于亲水性蛋白。多序列比对分析表明,两基因序列的相似性为98.7%。原核表达分析表明,这2个基因编码区能正常表达出目的蛋白,说明得到的这2个基因为功能基因而非假基因。
DREBs are a type of plant-specific transcription factor that plays an important regulatory role in abiotic stress. In this study, two DREB2 transcription factor genes Ss DREB2-1 and Ss DREB2-2 were cloned by homologous cloning using GenBank accession No. GenBank accession numbers KU963264 and KU963265, respectively. Sequence analysis showed that the lengths of these two genes were 814 bp and 709 bp, encoding 262 and 227 amino acids respectively. The predicted molecular weights of the two genes were 28.66 kD and 24.95 kD, respectively. The isoelectric points (PI) were 5.42 and 6.47. Amino acid hydrophilic / hydrophobic analysis showed that these two transcription factors belong to hydrophilic protein. Multiple sequence alignment analysis showed that the similarity of the two gene sequences was 98.7%. Prokaryotic expression analysis showed that the two gene coding region can express the target protein, indicating that the two genes obtained as functional genes rather than pseudogenes.