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秸秆直接还田会给作物生长带来不利影响,在还田过程中补加纤维素降解菌剂是解决其不利影响的有效方法.通过平板初筛及酶活综合测定及秸秆降解率测定复筛,从腐烂秸秆、牛粪堆肥、森林土等样品中筛选到5株高效纤维素降解菌,其中X1菌的酶活最佳,酶系组成最合理,其Cx,Cb,C1和FPA分别达到53.6 U/mL、69.7 U/mL、9.5 U/mL和11.6 U/mL,对秸秆的降解率达40.2%.进一步进行菌种组合,得到了一组秸秆降解率有明显增加的复合菌X1+X2,其降解率达到45.5%.根据X1和X2的菌落及个体形态特征,初步判定其均为青霉菌.
Straw directly into the field will have an adverse impact on crop growth, in the process of adding cellulose degradation bacteria is an effective way to solve its adverse effects. Five strains of cellulose-degrading bacteria were screened from samples of rotten straw, cow manure compost and forest soil. Among them, X1 strain had the best enzyme activity and the most reasonable enzyme composition, with Cx, Cb, C1 and FPA reaching 53.6 The biodegradation rate of straw was 40.2%, and the degradation rate of straw was 40.2%, 69.7 U / mL, 9.5 U / mL, and 11.6 U / mL, respectively. , And its degradation rate reached 45.5% .According to the morphological characteristics of colonies and individuals of X1 and X2, it was initially determined that all of them were Penicillium.