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目的:观察芪蓝颗粒对大鼠舌黏膜癌变模型淋巴细胞亚群的影响,并探讨其阻癌抑癌的免疫学机制。方法:SD大鼠150只,随机分为五组(A、B、C、D、E组),A、B、C、D组以4NQO饮水喂养诱导大鼠舌黏膜癌变,A组不作干预,作为阳性对照;B、C、D组分别给予不同剂量的芪蓝颗粒干预癌变过程,9、18、27、36周取舌标本进行组织病理学观察,并取新鲜全血,流式细胞术检测各样本全血中CD3+细胞、CD4+T细胞、CD8+T细胞的百分比,CD4/CD8比值及NK细胞。E组作为正常对照组。结果:多元方差分析表明,各组及各病理分级的CD3+细胞、CD4+T细胞百分比存在显著性差异(P<0.05)。组间比较,芪蓝颗粒干预组的CD3+、CD4+水平介于正常组与癌变模型组之间,统计表明:E组>C组>A组(P<0.05)。结论:芪蓝颗粒有阻癌抑癌作用,能降低致癌剂对机体免疫系统的影响,稳定CD3细胞水平,主要是通过稳定CD4+辅助性T细胞的水平,增强机体的防癌能力。
OBJECTIVE: To observe the effect of Qilan Granule on lymphocyte subsets in rat tongue mucosa canceration model and to investigate its immunological mechanism of tumor suppressor and tumor suppressor. Methods: One hundred and fifty SD rats were randomly divided into five groups (A, B, C, D and E). Groups A, B, C and D were fed with 4NQO drinking water to induce carcinogenesis of tongue mucosa in rats. As a positive control; Group B, C, D were given different doses of Qilan Granules intervention of carcinogenesis, tongue samples were taken at 9, 18, 27, 36 weeks histopathological observation, and fresh whole blood, flow cytometry The percentage of CD3 + cells, CD4 + T cells, CD8 + T cells, CD4 / CD8 ratio and NK cells in whole blood of each sample. E group as a normal control group. Results: Multivariate analysis of variance showed that the percentages of CD3 + cells and CD4 + T cells in each group were significantly different (P <0.05). The levels of CD3 + and CD4 + between intervention group and Qilan Granule intervention group were in the range of normal group and canceration model group. The statistics showed that: E group> C group> A group (P <0.05). CONCLUSION: Qilan Granule has the function of inhibiting cancer and inhibiting tumor, reducing the influence of carcinogenic agents on the immune system and stabilizing the level of CD3 cells, mainly through stabilizing the level of CD4 + helper T cells and enhancing the anti-cancer ability of the body.