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采用一阶导数光谱法测定310～290nm波长范围内的最大振幅值,计算胆酸含量,可消除干扰、本法简便、易行、灵敏度高、重现性好。
The first-order derivative spectroscopy was used to determine the maximum amplitude value in the wavelength range of 310-290nm. The content of cholic acid was calculated and the interference was eliminated. This method is simple, easy, sensitive and reproducible.