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目的对分布于我国及缅甸、老挝30个居群的珠子草进行遗传多样性分析,为珠子草遗传连锁图谱构建、种质资源保存及遗传育种等研究提供一定的理论依据。方法采用ISSR分子标记技术,对采自我国云南、广西、广东、福建、海南及缅甸、老挝共30个珠子草居群进行多态性和聚类分析。结果从60个ISSR引物中筛选出23条进行扩增,共扩增得到条带158条,其中共有条带43条,多样性条带115条,多态位点百分率(PPB)为72.78%,Nei’s基因多样性(H)为0.483 2,Shannon’s多态性信息指数(I)为0.676 0,多态性较高。结论我国珠子草居群大致分为沿海及内陆两大类,个别居群呈现明显的居群特异性,种质资源遗传多样性较高。
OBJECTIVE: To analyze the genetic diversity of Beauveria bassiana distributed in China, Burma and Laos, and to provide some theoretical basis for the establishment of genetic linkage map of Beauveria coli, germplasm conservation and genetic breeding. Methods The ISSR molecular markers were used to analyze the polymorphism and cluster analysis of 30 Beadgrass populations collected from Yunnan, Guangxi, Guangdong, Fujian, Hainan, Myanmar and Laos in China. Results A total of 23 bands were screened out from 60 ISSR primers. A total of 158 bands were found, of which there were a total of 115 bands with a diversity of 115 bands. The percentage of polymorphic bands (PPB) was 72.78% Nei’s gene diversity (H) was 0.483 2, Shannon’s polymorphism information index (I) was 0.676 0, and the polymorphism was high. Conclusion The population of Beadgrass can be roughly divided into two major categories: coastal and inland, with individual population showing obvious population specificity and high germplasm resources genetic diversity.