无血清培养是一项具有实用意义的研究课题,其应用范围也与日俱增。我们已成功地建立了V—Sis癌基因转化细胞的无血清培养系统,用以研究V—Sis癌基因转化细胞对生长因子的需求,并从条件培养液中分析其产物P_(28)~(V-sis)。培养液的基本组成如下:以DMEM/Ham′s F 12(1/1)作为基础培养基,各种添加物分别为胰岛素(2μg/ml)、转铁蛋白(5μg/mg)、 Serum-free culture is a practical research topic, its application is also growing. We have successfully established a serum-free culture system for V-Sis oncogene-transformed cells to study the growth factor requirement of V-Sis oncogene-transformed cells and analyzed the production of P_ (28) ~ ( V-sis). The basic composition of the culture medium was as follows: DMEM / Ham’s F12 (1/1) was used as a basal medium, and each of the supplements was insulin (2 μg / ml), transferrin (5 μg /