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肿瘤的快速生长依赖于新生血管的形成。血管内皮生长因子 (VEGF)是血管发生和形成过程中的主要介质 ,其特异性受体在正常组织和肿瘤组织的表达率存在数个数量级的差异 ,因此可以将毒素分子转运至增生的肿瘤上皮组织中抑制肿瘤血管增生 ,从而抑制肿瘤的生长。将白喉毒素的前 3 89个氨基酸基因片段与VEGF165通过一短肽相连构建为融合蛋白基因 ,在大肠杆菌中表达 ,获得纯化蛋白。实验证实该融合蛋白对血管内皮细胞有特异性杀伤作用 ,并研究了其对鸡胚尿囊膜新生血管的抑制作用
Rapid tumor growth depends on neovascularization. Vascular endothelial growth factor (VEGF) is a major mediator of angiogenesis and development, and its specific receptor has several orders of magnitude difference in the expression rate of normal and tumor tissues. Therefore, it is possible to transport toxin molecules to proliferating tumor epithelium Tissue inhibition of tumor angiogenesis, thereby inhibiting tumor growth. The first 3 89 amino acid fragments of diphtheria toxin and VEGF165 were linked by a short peptide to construct a fusion protein gene and expressed in E. coli to obtain the purified protein. Experiments confirmed that the fusion protein has a specific killing effect on vascular endothelial cells, and its inhibition of chick embryo allantoic membrane neovascularization