疟原虫进入肝实质内是其生活史中一个不可缺少的阶段。鉴于间日疟和卵形疟可于初次感染后数月复发,因此,研究抗疟药对肝期疟原虫的作用有重要意义。子孢子由吸血感染猴疟原虫或诺氏疟原虫的恒河猴的蚊内取得。猴疟原虫巴氏亚种和猴疟原虫柬埔寨株的子孢子源于斯氏和德氏按蚊。诺氏疟原虫H株的子孢子从德氏按蚊内分离。按蚊血餐12～22天后,无菌解剖 Plasmodium into the liver parenchyma is an indispensable stage in their life history. In view of the vivax malaria and oval malaria can be relapsed in the first few months after the first infection, therefore, the study of anti-malarial drugs on liver stage malaria parasite is of great significance. Sporozoites are harvested from mosquitoes of rhesus macaques sucking Plasmodium or Plasmodium. Sporozoites of Plasmodium vivax and Plasmodium malaria Cambodian strains originate from Anastrephans inns and Anopheles stephensi. Sporozoites of P. nidulans H strain were isolated from An. Mosquito. Anopheles blood meal 12 to 22 days after sterile dissection