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研究β-L－2’，3’-双脱氧-5-氟胞苷的抗乙型肝炎病毒作用。方法：从2．2．15细胞培养液中提取乙肝病毒DNA，用DNA印迹法分析药物对乙肝病毒DNA复制的影响。观察药物对人T-成淋巴样细胞的生长抑制作用，用狭线印迹法检测人T-成淋巴样细胞的线粒体DNA。结果：β-L-2’，3’-双脱氧-5-氟胞苷对乙肝病毒DNA合成的半数抑制浓度为0．05μmol／L，但其抗乙肝病毒作用是可逆的。对人T-成淋巴样细胞的半数生长抑制浓度为67μmol／L。选择指数远高于2’，3’-双脱氧胞苷。在浓度高到足以抑制乙肝病毒复制达90％以上时，对细胞线粒体DNA合成并无影响。结论：β-L-2’，3’-双脱氧-5-氟胞苷在体外有较强的选择性抗乙肝病毒作用。
To study the anti-hepatitis B virus effect of β-L-2 ’, 3’-dideoxy-5-fluorocytidine. Methods: Hepatitis B virus DNA was extracted from 2.2.15 cell culture medium and the effect of drugs on HBV DNA replication was analyzed by Southern blotting. The inhibitory effect of the drug on the growth of human T-lymphoblastoid cells was observed, and the mitochondrial DNA of human T-lymphoblastoid cells was detected by a slit-blotting method. Results: The half inhibitory concentration of β-L-2 ’, 3’-dideoxy-5-fluorocytidine on HBV DNA synthesis was 0.05μmol / L, but its anti-HBV effect was reversible. The half inhibitory concentration of human T-lymphoblastoid cells was 67 μmol / L. The selection index is much higher than 2 ’, 3’-dideoxycytidine. At concentrations high enough to inhibit hepatitis B virus replication by more than 90%, there is no effect on cellular mitochondrial DNA synthesis. Conclusion: β-L-2 ’, 3’-dideoxy-5-fluorocytidine has strong selective anti-HBV effect in vitro.