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目的探讨凝血酶敏感蛋-1(TSP-1)、血管内皮细胞生长因子(VEGF)、微血管密度(MVD)在判断骨肉瘤患者复发转移中的价值。方法采用免疫组织化学法检测65例骨肉瘤手术切除标本的VEGF、TSP-1蛋白的表达情况并计数MVD,对上述指标与术后复发转移率之间的关系进行回顾性分析。结果全组病例TSP-1、VEGF蛋白表达阳性率分别为30.77%(20/65)和61.54%(40/65);MVD平均值为(23.68±9.42)/200倍视野;VEGF阳性组的术后复发转移率高于VEGF阴性组(P<0.05);TSP-1阳性组的术后复发转移率低于TSP-1阴性组(P<0.05);多因素分析表明,MVD和淋巴结状况为判断骨肉瘤术后复发转移的独立因素(P<0.05)。结论MVD是判断骨肉瘤术后复发转移的独立因素,具有判断术后复发转移的风险及预后的价值。
Objective To investigate the value of TSP-1, VEGF and MVD in determining the recurrence and metastasis of osteosarcoma. Methods Immunohistochemistry was used to detect the expression of VEGF and TSP-1 protein in 65 cases of osteosarcoma. The relationship between the above indexes and recurrence and metastasis was retrospectively analyzed. Results The positive rates of TSP-1 and VEGF in all the cases were 30.77% (20/65) and 61.54% (40/65), respectively. The mean MVD was (23.68 ± 9.42) / 200 times of field of vision. The VEGF-positive group (P <0.05). The recurrence and metastasis rate of TSP-1 positive group was lower than that of TSP-1 negative group (P <0.05). Multivariate analysis showed that MVD and lymph node status Osteosarcoma recurrence and metastasis after the independent factors (P <0.05). Conclusions MVD is an independent factor to judge the recurrence and metastasis of osteosarcoma, which has the value of judging the risk and prognosis of recurrence and metastasis.