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目的:研究白细胞介素10(IL-10)基因对链脲佐菌素(STZ)诱导的糖尿病大鼠胰腺炎症浸润程度及胰腺组织中Bcl-2及Bax表达的影响。方法:建立链脲佐菌素性糖尿病模型,腺病毒介导的IL-10基因(Ad-mIL-10)腹腔注射。检测大鼠空腹血糖值;免疫组织化学法观察胰腺炎症浸润程度;TUNEL法检测胰岛细胞凋亡;免疫组化方法观察Ad-mIL-10对实验性糖尿病大鼠胰岛凋亡调控基因Bax和Bcl-2表达的影响。结果:Ad-mIL-10腹腔注射糖尿病发病率低,平均血糖水平低,可以降低胰腺炎症浸润程度,减少胰岛细胞凋亡。给予Ad-mIL-10后大鼠Bax基因的表达明显下降,Bcl-2与Bax的比值明显增加。结论:IL-10基因对实验性糖尿病大鼠有降血糖作用,减少胰岛细胞凋亡,与调节Bcl-2与Bax基因的表达有关。
Objective: To investigate the effect of interleukin 10 (IL-10) gene on infiltration of pancreatic inflammation and the expression of Bcl-2 and Bax in pancreatic tissues of streptozotocin-induced diabetic rats. Methods: Streptozotocin-induced diabetic model and adenovirus-mediated intraperitoneal injection of IL-10 gene (Ad-mIL-10) were established. The levels of fasting blood glucose in rats were measured by immunohistochemistry and the infiltration of inflammatory cells in pancreatic tissues were observed by immunohistochemical method. The apoptosis of pancreatic islet cells was detected by TUNEL method. The expressions of Bax and Bcl- 2 expression. Results: Ad-mIL-10 intraperitoneal injection of low incidence of diabetes, the average low blood sugar levels, can reduce the infiltration of pancreatic inflammation, reduce pancreatic islet cell apoptosis. The expression of Bax gene in Ad-mIL-10 rats decreased significantly, and the ratio of Bcl-2 to Bax increased significantly. Conclusion: IL-10 gene has hypoglycemic effect on experimental diabetic rats and reduces the apoptosis of islet cells, which is related to the regulation of Bcl-2 and Bax gene expression.