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为进一步优化烟草施肥方案,采用大田试验,研究了不同施氮水平(0、37.5、75.0、112.5、150 kg)下云南地区主栽烟草品种(K326、NC297、红花大金元)的碳氮代谢关键酶活性。结果表明,随氮素水平的提高同一品种烟草的硝酸还原酶、蔗糖转化酶、淀粉酶活性均提高;但在成熟期,增加施氮量后,烟草的淀粉酶活性反而降低;低氮水平(0、37.5 kg/hm2)下3个烟草品种的硝酸还原酶、蔗糖转化酶、淀粉酶活性均较低,不利于烟株生长;高氮(150 kg/hm2)水平下,烟株易长势过旺,黑爆青筋。不同烟草品种在某些相同施氮水平下的碳氮代谢酶有显著差异,但总体来看,中氮水平(75.0、112.5 kg/hm2)能适时转化碳氮代谢,满足优质烟叶的生长要求。
In order to further optimize the tobacco fertilization scheme, the field trials were conducted to study the effects of carbon and nitrogen of main tobacco varieties (K326, NC297 and Honghua Daijin) under different nitrogen levels (0, 37.5, 75.0, 112.5 and 150 kg) Metabolic key enzyme activity. The results showed that the nitrate reductase, sucrose invertase and amylase activities of the same tobacco variety increased with the increase of nitrogen level. However, at the mature stage, the amylase activity of tobacco decreased but the nitrogen level decreased 0,37.5 kg / hm2), the nitrate reductase, sucrose invertase and amylase activities of the three tobacco cultivars were low, which were unfavorable for the growth of tobacco plants. Under high nitrogen (150 kg / hm2) Wang, black burst blue tendons. There were significant differences in the carbon and nitrogen metabolism enzymes between different tobacco cultivars at some of the same N application rates. However, on the whole, the medium N level (75.0,112.5 kg / hm2) could be converted to carbon and nitrogen metabolism in time to meet the growth requirements of high quality tobacco.