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目的建立安尔眠胶囊中丹参素钠含量的测定方法。方法采用高效液相色谱(HPLC)法对安尔眠胶囊中的丹参素钠进行含量测定。色谱柱为Hypersil ODS2(250 nm×4.6 nm,5μm),流动相为甲醇-1.1%冰醋酸(5∶95),流速为1.0 mL/min,检测波长为281 nm。结果丹参素钠对照品在2 1.44~214.40μg/mL范围内呈良好的线性关系(r=0.999 9,n=6),平均加样回收率为98.78%,RSD为2.15%(n=6)。结论本法操作简便,结果可靠,重现性好,适用于安尔眠胶囊的质量控制。
Objective To establish a method for the determination of sodium Danshensu in Anlian Capsule. Methods The content of Danshensu Sodium in Ansalmian Capsules was determined by high performance liquid chromatography (HPLC). The chromatographic column was Hypersil ODS2 (250 nm × 4.6 nm, 5 μm). The mobile phase was methanol-1.1% glacial acetic acid (5:95) at a flow rate of 1.0 mL / min with a detection wavelength of 281 nm. Results The sodium dansyl sulfate showed a good linearity (r = 0.999 9, n = 6) in the range of 2.144 ~ 214.40μg / mL with the average recovery of 98.78% and RSD of 2.15% (n = 6) . Conclusion This method is simple, reliable, reproducible, suitable for the quality control of An Er-Mian Capsule.