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为研究幽门螺杆菌（ＨＰ）球形变异本质，作者通过延期培养和采用亚抑菌浓度抗生素，使３株ＨＰ发生球形变异，对弯曲形和球形ＨＰ作了ＳＤＳ－ＰＡＧＥ、免疫印迹及４个毒力基因片段ＰＣＲ和ＰＣＲ－ＳＳＣＰ分析。ＳＤＳ－ＰＡＧＥ图谱显示球形ＨＰ分子量在７４×１０４以上的蛋白含量减少，免疫印迹显示球形ＨＰ１２５×１０４蛋白条带反应减弱，而抗生素诱变的球形ＨＰ分子量为１１×１０４和６３×１０４的蛋白条带反应增强。ＰＣＲ及ＰＣＲ－ＳＳＣＰ结果表明球形ＨＰ的ｈｐａＡ，ＶａｃＡ，ＣａｇＡ和ＵｒｅＡ４个毒力基因片段未发生缺失，但在ｈｐａＡ或ＶａｃＡ基因中存在点突变
In order to study the nature of the spherical aberration of Helicobacter pylori (HP), the three strains of HP were spherically characterized by deferred culturing and using antibiotics at sub-bacteriostatic concentrations. SDS-PAGE, immunoblot and four toxic Force gene fragment PCR and PCR-SSCP analysis. SDS-PAGE showed that the molecular weight of globular HP was reduced more than 7.4 × 104, and the immunoprecipitation showed that the banding reaction of globular HP12 × 5 × 104 protein was weakened, while the antibiotic-induced globular HP molecular weight was 11 × 104 and 61. 3 × 104 protein band reaction enhanced. The results of PCR and PCR-SSCP showed that there were no deletions in four virulence genes of hpaA, VacA, CagA and UreA in globular HP, but there were point mutations in hpaA or VacA genes