用高分辨电镜方法研究蛋白质晶体结构有其独到之处,而获得合乎分析用的蛋白质薄晶,并保存好其结构,是关键性问题之一。过氧化氢酶用NaCl透析法获得。酶溶于10％NaCl,pH5.7,使其浓度为10mg/ml,于4℃,依蒸馏水透析。枯草杆菌2—淀粉酶溶于pu9-10NH_4·H_2O,使酶浓度为10mg/ml,于室温对20％叔丁醇透析即得其薄晶。 Using high-resolution electron microscopy to study the crystal structure of proteins has its own uniqueness. To obtain protein thin crystals suitable for analysis and preserve its structure is one of the key issues. Catalase is obtained by dialysis with NaCl. The enzyme was dissolved in 10% NaCl, pH 5.7 to a concentration of 10 mg / ml and dialyzed against distilled water at 4 ° C. Bacillus subtilis 2-amylase was dissolved in pu9-10NH 4 · H 2 O so that the enzyme concentration was 10 mg / ml, and the thin crystal was obtained by dialyzing 20% ​​tert-butanol at room temperature.