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目的:探讨橄榄苦苷(OE)对大鼠局灶性脑缺血/再灌注损伤和炎性反应的作用。方法:雄性SD大鼠随机分为假手术组(Sham组)、溶媒处理组(Vehicle组)和OE处理组(OE组)。用线栓法制作大脑中动脉脑缺血/再灌注(MCAO/R)模型。TTC染色检测脑梗死体积,免疫组化法检测缺血侧大脑皮层髓过氧化物酶(myeloperoxidase,MPO)和肿瘤坏死因子(TNF-α)表达,Western Blot法检测基质金属蛋白酶-9(matrix metallopeptidase 9,MMP9)及基质金属蛋白酶-2(matrix metallopeptidase 2,MMP2)的表达。结果:(1)Vehicle组大脑缺血侧有明显的梗死灶,而OE处理组脑梗死体积明显有所缩小(P<0.01)。(2)Vehicle组MPO和TNF-α表达与Sham组相比显著提高(P<0.01),而OE组两者的表达较Vehicle组明显降低(P<0.01,P<0.05)。(3)Western Blot显示:Vehicle组MMP2、MMP9的表达水平较Sham组显著提高,而OE处理下调两者表达(P<0.01,P<0.05)。结论:OE可能通过抑制炎性反应保护脑缺血再灌注损伤。
Objective: To investigate the effect of oleuropein (OE) on focal cerebral ischemia / reperfusion injury and inflammatory reaction in rats. Methods: Male SD rats were randomly divided into Sham group, vehicle group and OE group. Middle cerebral artery occlusion (MCAO / R) model was made by thread occlusion. The volume of cerebral infarction was detected by TTC staining, the expression of myeloperoxidase (MPO) and tumor necrosis factor (TNF-α) in ischemic cortex were detected by immunohistochemistry. The expression of matrix metallopeptidase 9, MMP9) and matrix metallopeptidase 2 (MMP2). Results: (1) There was obvious infarction on the ischemic side of Vehicle group in Vehicle group, while the volume of infarction in OE group was significantly reduced (P <0.01). (2) Compared with Sham group, the expression of MPO and TNF-α in Vehicle group were significantly increased (P <0.01), while those in OE group were significantly lower than those in Vehicle group (P <0.01, P <0.05). (3) Western Blot showed that the expressions of MMP2 and MMP9 in Vehicle group were significantly increased compared with those in Sham group, while those in OE group were down-regulated (P <0.01, P <0.05). Conclusion: OE may protect cerebral ischemia-reperfusion injury by inhibiting inflammatory reaction.