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目的 细胞因子ＴＮＦ、ＩＦＮ等在淋巴细胞介导胶质瘤细胞调亡方面的作用和特点。方法 采用免疫 组化、分子生物学、ＭＴＴ法及ＰＢＬ对培养的胶质瘤细胞凋亡形成的直接观察方法。结果 正常人ＰＢＬ对Ｕ－ ２５１ＭＧ的杀伤活性明显高于ＴＮＦ或ＩＦＮ的单独作用，ＩＦＮ＋ＰＢＬ组对Ｕ－２５１ＭＧ的杀伤作用较 ＰＢＬ及 ＴＮＦ＋ ＰＢＬ组的杀伤作用凋亡细胞很少而核分裂相细胞多；ＰＢＬ＋ＩＦＮ组出现大量肿瘤细胞凋亡。结论 细胞凋亡调节 失常是慢性增殖性肿瘤形成并发展的主要原因。从免疫活性细胞可以通过促凋亡方式抗肿瘤以来，如何通过调节 免疫系统预防和治疗肿瘤已成为目前研究的热点。认为ＭＴＴ法检测ＰＢＬ介导胶质瘤细胞凋亡有特殊意义。在淋 巴细胞抗肿瘤过程中介导释放ＩＦＮ使肿瘤细胞ＴＮＦＲ及ＦａｓＲ表达增强，同时通过胞浆中的穿孔素，颗粒酶的释 放导致肿瘤细胞凋亡。ＴＮＦ引起的肿瘤杀伤作用是与ＴＮＦＲ相关的ＦａｓＲ介导的。ＦａｓＬ及抗Ｆａｓ抗体与Ｆａｓ集合 后导致细胞凋亡。
Objective cytokines such as TNF, IFN in lymphocyte-mediated glioma cell apoptosis in the role and characteristics. Methods Immunohistochemistry, molecular biology, MTT and PBL were used to directly detect the apoptosis of cultured glioma cells. Results The cytotoxicity of PBL to U-251MG was significantly higher than that of TNF or IFN alone. The cytotoxicity of IFN-γ against PBMC was higher than that from PBL and TNF-α PBL. PBL + IFN group appeared a large number of tumor cell apoptosis. Conclusion The abnormal regulation of apoptosis is the main reason for the formation and development of chronic proliferative tumors. Since immunocompetent cells can be anti-tumor through pro-apoptotic methods, how to prevent and treat tumors by regulating the immune system has become a hot research topic. MTT assay that PBL-mediated glioma cell apoptosis has special significance. Mediated release of IFN during lymphocyte anti-tumor therapy enhances the expression of TNFR and FasR in tumor cells, meanwhile, the release of granzyme in peritumoral cells leads to tumor cell apoptosis. Tumor-causing effects of TNF are TNFR-related FasR-mediated. FasL and anti-Fas antibody together with Fas lead to apoptosis.