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为加快AL型杂交小麦的发展,以不育系AL18A、恢复系99AR144-1及二者杂交F2代群体为材料,选用SSR标记和分离群体分组分析法进行育性恢复基因的QTL定位。结果表明,育性恢复由主效和微效基因共同控制,采用复合区间作图法分析,在1B染色体上检测到了1个主效恢复基因QTLqRf-1B-1,在5AL染色体上检测到了1个微效QTLqRf-5A-1。qRf-1B-1位于SSR标记Xbarc8与Xgwm413之间,与两标记的遗传距离分别为0.85cM和2.00cM,LOD值为14.06,加性效应为18.87,可解释22.43%的表型变异;qRf-5A-1位于SSR标记Xgwm595与Xgwm410之间,与两标记的遗传距离分别为10.00cM和0.10cM,LOD值为3.18,加性效应为12.32,可解释5.44%的表型变异。
In order to speed up the development of AL type hybrid wheat, the QTLs for fertility restorer gene were selected by SSR markers and segregation analysis of segregating population in F2 generation of male sterile line ALARIAA, restorer line 99AR144-1 and their crosses. The results showed that the fertility restoration was controlled by both major and minor genes. Using composite interval mapping method, QTLqRf-1B-1 was detected on 1B chromosome and 1 on 5AL chromosome Mimic QTLqRf-5A-1. qRf-1B-1 was located between SSR markers Xbarc8 and Xgwm413. The genetic distance between the two markers was 0.85cM and 2.00cM, the LOD value was 14.06, the additive effect was 18.87, which explained 22.43% of phenotypic variation. qRf- 5A-1 was located between SSR markers Xgwm595 and Xgwm410. The genetic distances to the two markers were 10.00cM and 0.10cM, respectively. The LOD value was 3.18 and the additive effect was 12.32, which explained 5.44% of the phenotypic variation.