以适当参数的ＮｄＹＡＧ激光微束切割大麦７Ｈ染色体后再利用微细玻璃针挑取了７ＨＳ端部片段并放入Ｅｐｐｅｎｄｏｒｆ管中，建立了一种激光微束与玻璃针结合使用微切割、微分离植物染色体片段的方法，为今后植物染色体特定区域ＤＮＡ的微克隆技术提供了一种新的方法 The 7H chromosome of barley was cut with the NdYAG laser microbeam with appropriate parameters, and then the 7HS end fragment was picked up by micro glass needle and put into Eppendorf tube. A laser microbeam combined with glass needle was used to establish microdissection, Chromosomal fragments of the method for the future plant chromosome specific regions of DNA micro-cloning technology provides a new method