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目的：观察恙虫病疫区恙螨的季节消长，分离病原体。方法：捕捉活鼠，捡螨；螨的生理盐水悬液，腹腔接种小鼠，取发病者肝、脾、肾制印片或腹膜涂片，镜检恙虫病立克次体（Ｒｔ）；采用ＩＦＡ法进行血清学检测与分型。结果：从鼠体外共捡获１１７６２只恙螨，其中小盾纤恙螨（Ａ）２５４７只（占２１６５％），须纤恙螨（Ｂ）１５９６只（占１３５７％），临淮岗纤恙螨（Ｃ）２８３３只（占２４０９％），泰山纤恙螨（Ｄ）４６６只（占３９６％），太平洋无前恙螨（Ｅ）４３２０只（占３６７３％）。恙螨季节变化：Ａ主要出现在９～１２月，高峰为１１月，是秋季鼠体外绝对优势种；Ｂ出现在１０月至翌年４月，高峰为１２月，是冬季优势种；Ｃ于５月出现，１１月消失，高峰为８月，是夏季优势种；Ｄ主要出现在６～１０月，数量较少，且起伏较大；Ｅ全部采自大仓鼠，主要出现在４～１２月，高峰为７月。Ｒｔ分离：Ａ共接种１０批，分离到４株Ｒｔ；Ｂ、Ｃ、Ｅ各接种４批，分离到４株、２株和２株Ｒｔ。血清学分型：除Ｃ分离到１株为Ｋａｒｐ型外，其余均为Ｇｉｌｉａｍ型。结论：Ａ是主要传播媒介，Ｒｔ流行型别以Ｇｉｌｉａｍ型为主
Objective: To observe the seasonal fluctuation of chigger mites in the epidemic area of tsutsugamushi disease and isolate pathogens. Methods: Catching live mites and picking up mites; Mite saline suspension was intraperitoneally inoculated into mice and liver, spleen, kidney imprints or peritoneal smears were taken from the patients and Rickettsia tsutsugamushi (Rickettsia tsutsugamushi) IFA method for serological detection and typing. Results: A total of 11762 chigger mites were collected from rats in vitro. Among them, 2547 chickens (A), 2547 (2165%), 1596 chickens (1357%), 2833 (29.09%) of the chigger mites (C), 466 (396%) of the chigger mites (D) in the Taishan Mountains, and 4320 (43.7%) in the Pacific without chigger mites (E) %). Seasonal changes of chigger mites: A mainly appeared in September-December and peaked in November, which was the absolute dominant species in autumn; B appeared in October-April of the following year with a peak of December, which was the dominant species in winter; C was 5 Month, disappeared in November, peaked in August, is the dominant species in summer; D mainly occurs in June to October, the number is small, and the ups and downs; E collected from large hamsters, mainly in April to December, The peak is July. Rt separation: A total of 10 batches were inoculated, 4 Rt were isolated; 4, 2, and 2 Rt were isolated from each of B, C and E inoculation. Serological typing: In addition to the C strain isolated Karp type, the rest are Giliam type. Conclusion: A is the main media, and the prevalence of Rt is Giliam type