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为了研究荞麦属不同种间植物脱落酸不敏感基因(ABA-insensitive,ABI)序列变异差异与亲缘关系,试验选用7个荞麦野生种,包括野甜荞、野苦荞、左贡野荞、毛野荞、大野荞、金荞麦、硬枝万年荞共30份种质材料,提取苗期幼嫩叶片的DNA,通过聚合酶链式反应(polymerase chain reaction,PCR)扩增获得荞麦ABI基因,并对扩增片段进行基因测序与序列分析。结果发现,7个野生种共30份种质间ABI基因片段的多态位点数占20.28%,其中,14份野苦荞间ABI基因片段多态位点数占1.86%,5份野甜荞间多态位点数占1.81%,说明荞麦属植物种内ABI基因序列高度保守。对扩增序列进行遗传距离分析发现,左贡野荞与毛野荞种间遗传距离最大,硬枝万年荞与金荞麦种间遗传距离最小。聚类分析结果显示:野苦荞与大野荞、金荞麦和硬枝万年荞亲缘关系较近,与毛野荞亲缘关系次之;左贡野荞与野甜荞亲缘关系近;不同地区野苦荞和左贡野荞分别被单独聚为一类,说明生长环境对荞麦ABI基因序列变异具有影响。
In order to study the sequence variation and genetic relationship of abscisic acid (ABA) insensitive gene (ABI) in different species of buckwheat, seven buckwheat wild species were selected, including wild sweet buckwheat, wild buckwheat, Zuo Gong wild buckwheat, , Wild buckwheat, golden buckwheat, hardwood buckwheat were collected from 30 germplasm materials, and the DNA of young leaves at seedling stage was extracted. The ABI gene of buckwheat was amplified by polymerase chain reaction (PCR) Amplify fragments for gene sequencing and sequence analysis. The results showed that there were 20.28% polymorphic loci in ABI gene fragments of 30 germplasm among 7 wild species, of which 14 loci of ABI gene fragments accounted for 1.86%, 5 wild sweet buckwheat The number of polymorphic loci accounted for 1.81%, indicating that the sequence of ABI gene in buckwheat species is highly conserved. Genetic distance analysis of amplified sequences showed that the genetic distance between Zuo Gong Ye Qiu and Mao Yuan Buckwheat was the largest, and the genetic distance between Qiu Wan Qiao Wan and F buckle was the smallest. The results of cluster analysis showed that wild bitter buckwheat was closely related to the wild buckwheat, golden buckwheat and hardwood Wannian buckwheat, followed by Phyllostachys pubescens. The genetic relationship between wild buckwheat and wild sweet buckwheat was close. Zuo Gong wild buckwheat were individually grouped together, indicating that the growth environment of buckwheat ABI gene sequence variation has an impact.