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经插入水底的带多孔喷头导管，将臭氧通入水中。向１２ ｃｍ 内径玻璃瓶装３ Ｌ含菌蒸馏水连续加臭氧１０ ｍｉｎ（１０－５６ ｍｇ） ，对大肠杆菌和金黄色葡萄球菌杀灭率均为１００ ％ ，连续加臭氧１２０ ｍｉｎ 对枯草杆菌黑色变种芽胞杀灭率亦达１００ ％ 。向３０ ｍｌ ＨＢｓＡｇ 悬液中连续加臭氧１５ ｍｉｎ（１１－８８ ｍｇ） ，可将ＨＢｓＡｇ 抗原性完全破坏。其杀菌效果受有机物影响较大。
After inserting the bottom of the tube with a porous nozzle, the ozone into the water. To 12 cm diameter glass bottle 3 L of distilled water containing bacteria plus ozone 10 min (10-56 mg), the killing rate of E. coli and Staphylococcus aureus were 100% continuous addition of ozone 120 min Bacillus subtilis black mutant spores Kill rate also reached 100%. Continuous addition of ozone to 30 ml HBsAg suspension for 15 min (11-88 mg) completely destroyed HBsAg antigenicity. Its bactericidal effect is greatly affected by organic matter.